I would recommend a defrag of teh catalog volume.
We defrag out catalog volume daily (Mon-Fri) between 14:00 and 16:00 - when backups are usually not running, and the catalog backup and it's image db cleanup have completed usually - however, sometimes, backups overrun, and/or the catalog backup overrruns and/or image db cleanup overrun, and overlap with the defrag - and we've never had any issues in several years of running Raxco Perfect Disk (we currently use v8) - all on a clustered resource SAN attached (HSG replicated over twin fabrics). Also, we defrag the C: (system vol) and D: (NetBackup binaries) volumes weekly on Sunday's whilst backups are running.
Because it's Windows we occaisionally have to reboot during the day, and we never worry about whether defrag is runnign or not - for us Raxco Perfect Disk was a set and forget.
Catalog backup of a 150GB catalog (compressed using 7 day catalog compression from 150GB to 70GB sitting within a 100GB volume) is back down to an average duration of 3:30, but it did creep up to 6 hrs without defrags.
The daily defrag run takes about 45 to 60 minutes to pass over 150GB of data (70GB on disk) in a 100 GB NTFS volume.
Windows 2000 Adv Server SP4 MSCS (active/passive) cluster, running NBU ES v5.1 MP6, over twin fabrics.