When you backup dedup data to tape, the data is hydrated before it is backed up.
There is no problem in having more than 1 tape for a single backup. I normally try not to mix my LTO3 and LTO4 tapes. There are differences between the two types of tapes. For example, LTO4 support hardware encryption whereas LTO3 does not, so I don't know what would happen when the tape is spanned and hardware encryption is used. Also, the writing speed of the two types of tapes are different. Before you mix them, I would suggest that you thoroughly test them first, i.e., make sure that you have no problems restoring the data.