Yes you can do that:
To add a deduplication storage folder
1 On the navigation bar, click Devices.
2 In the task pane, under Device Tasks, click Configure devices assistant.
3 On the ConfigureDevicesAssistant dialog box, under DeduplicationOption,
click Deduplication Storage Folder.
4 If the Deduplication Storage Folder Configuration dialog box appears, click
Add a Deduplication Storage Folder.
This step does not apply if this is the first deduplication storage folder. The
Deduplication Storage Folder Configuration dialog box appears only if a
deduplication storage folder already exists on another media server.
5 Complete the options on the General tab.
6 Complete the options on the Advanced tab.
7 On the Sharing tab, select the media servers that you want to use with this
deduplication storage folder.
8 Click OK.
9 Restart the Backup Exec services on the media servers that you selected in
step 7.